NAD+
NAD+ is β-nicotinamide adenine dinucleotide, a pyridine dinucleotide coenzyme, with the molecular formula C21H27N7O14P2 and a molecular weight of 663.43 g/mol.
NAD+ is a coenzyme — a pyridine dinucleotide — not a peptide: it contains no amino acid residues and no peptide bonds.
Identity & structure
Verified identifiers, sequence, and analytical properties — every value machine-readable and independently sourceable against PubChem.
| Identity | |
|---|---|
| CAS | 53-84-9 |
| PubChem CID | 5892 |
| FDA UNII | 0U46U6E8UK |
| Also known as | NAD, NAD+, NAD plus, beta-NAD+, beta-NAD, Nicotinamide adenine dinucleotide, Nadide, Diphosphopyridine nucleotide, Coenzyme I, DPN |
| Research class | Secretagogue & Regulatory |
| Structure | |
| Molecular formula | C21H27N7O14P2 |
| InChIKey | BAWFJGJZGIEFAR-NNYOXOHSSA-N |
| Sequence | — |
| Length | Dinucleotide |
| Analytical | |
| Average molecular weight | 663.43 g/mol |
| Monoisotopic mass | 663.1091 Da |
| Form | Lyophilized powder |
| Solubility | Water-soluble |
| Storage | -20 °C, desiccated |
Where NAD+ sits in its class
Molecular weight (g/mol) across the 21 characterized compounds in Secretagogue & Regulatory. Compare all compounds →
Research context
NAD+ is β-nicotinamide adenine dinucleotide, a pyridine dinucleotide coenzyme, with the molecular formula C21H27N7O14P2 and a molecular weight of 663.43 g/mol. It is a characterized chemical reference material — not a peptide — and is supplied strictly for analytical and laboratory research use.
Supplied solely as a characterized research material. No representation is made as to any biological effect. For Research Use Only — not for human or veterinary use.
How NAD+ is identified
Identity and purity are measured, not asserted. Electrospray mass spectrometry (ESI-MS) determines the mass-to-charge ratio of the intact molecule — an observed value matching the expected series above confirms the molecular formula. Reversed-phase HPLC separates the sample over time, and purity is read as arithmetic: the target peak’s area as a share of total peak area.
Handling & stability
Physical form, storage, and solubility as supplied. Handling guidance only — no use protocols.
Related compounds
Other compounds in this research class.
Frequently asked questions
What is NAD+?
Is NAD+ a peptide?
What is the molecular formula of NAD+?
What is the CAS number of NAD+?
How is the identity of NAD+ confirmed?
How should NAD+ be stored?
Is NAD+ approved for human use?
Data, tools & certificates
Research literature
Research literature — for reference only
Curated peer-reviewed references indexing the identity, characterization, and analytical-method literature for NAD+, from the U.S. National Library of Medicine (PubMed). Listed as neutral citations only — no findings, conclusions, or use are stated or implied.
- An Improved Two-Dimensional HPLC Method for Endogenous 2'-Deoxy-ADPR and 2'-Deoxy-NAD.
- Quantifying the extended energy metabolome of industrially important microorganisms (Saccharomyces cerevisiae) using ultra-performance liquid chromatography with mass spectrometry.
- Simultaneous quantification of 26 NAD-related metabolites in plasma, blood, and liver tissue using UHPLC-MS/MS.
- Assays for Determination of Cellular and Mitochondrial NAD(+) and NADH Content.
- An Integrated LC-MS/MS Strategy for Quantifying the Oxidative-Redox Metabolome in Multiple Biological Samples.
- Measurement of Pyridine Nucleotides in Biological Samples Using LC-MS/MS.
- Quantifying the cellular NAD+ metabolome using a tandem liquid chromatography mass spectrometry approach.
- HPLC determination of oxidized and reduced pyridine coenzymes in human erythrocytes.
References
- PubChem — compound identity, structure & computed properties (CID 5892). PubChem →
- CAS Registry Number 53-84-9.
- FDA Global Substance Registration System — UNII 0U46U6E8UK.
Research professionals only
NAD+ — from the record to the bench
The monograph and every lot certificate stay public. Pricing and ordering open for verified research professionals.