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Method / concept

Molecular weight

Molecular weight is the mass of one molecule of a compound, reported in daltons (equivalently g/mol). It follows directly from the molecular formula, and it is the value a mass spectrometer is used to confirm — the quantitative half of a compound’s identity.

Definition. The mass of one molecule of a compound, reported in daltons (g/mol).

How it works

Because mass is additive, a molecule’s weight is the sum of the masses of its atoms, which is why it can be computed exactly from the formula. Two conventions are in use: the monoisotopic mass (each element’s most abundant isotope) and the average mass (weighted over natural isotope abundances).

The two figures differ, and mixing them is a common error. High-resolution mass spectrometry resolves the isotope pattern and is compared against the monoisotopic value; a lower-resolution or nominal figure is usually the average. A number should be read knowing which convention it follows.

Molecular weight is a property of the molecule, not of a lot. It does not change from batch to batch; what changes between lots is purity, which is measured separately.

Why it matters

Molecular weight is what makes identity checkable. ESI-MS measures a mass-to-charge ratio, and comparing the observed value against the weight computed from the formula is how identity is confirmed.

It is also a sanity check on the formula: an independently computed mass that disagrees with the stated weight flags an error in one of them — a check that has caught mistakes a name alone would pass.

Like the formula, it is necessary but not sufficient: isomers share a mass, so a matching weight confirms the mass but not the arrangement of atoms.

At Lineará

Each compound’s molecular weight is published on its compound page and, where applicable, on the certificate, in g/mol alongside the molecular formula.

The expected mass series a certificate’s identity check relies on is derived by construction and reconciled against an independently computed mass.

Frequently asked questions

What is molecular weight?
The mass of one molecule of a compound, reported in daltons (g/mol). It follows directly from the molecular formula.
What is the difference between monoisotopic and average mass?
Monoisotopic mass uses each element’s most abundant isotope; average mass is weighted over natural isotope abundances. High-resolution measurements are compared against the monoisotopic value, and the two should not be mixed.
Does molecular weight change between lots?
No. It is a property of the molecule. What changes between lots is purity, which is measured separately.
How is molecular weight confirmed?
By ESI-MS: the observed mass-to-charge ratio is compared against the weight computed from the molecular formula.

Research professionals only

Every term, on the record

Definitions are analytical and characterization-only, and trace back to the compounds and methods they describe.

áFor Research Use Only · Not for human or veterinary use · Not for diagnostic or therapeutic use